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The antibody is from purified rabbit serum by affinity purification by way of sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

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Overexpression of this gene continues to be observed in several tumors, which results in chromosome instability, and so could contribute to tumorigenesis. This protein was found to affiliate with, and become linked to, the phosphorylation of NPAT protein (nuclear protein mapped for the ATM locus), which participates in mobile-cycle controlled histone gene expression and performs a significant purpose in selling cell-cycle progression within the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode distinctive isoforms, happen to be described. Two more splice variants had been described but in-depth nucleotide sequence facts is not really nonetheless available.

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Blocking peptides are peptides that bind exclusively towards the concentrate on antibody and block antibody binding. These peptide ordinarily consists of the epitope identified through the antibody.

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This guideline outlines even further achievable optimization of mobile and immunoassay parameters to be sure the best possible success are...

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This guideline outlines more feasible optimization of mobile and immunoassay parameters to be certain the best possible benefits are...

Antibodies sure to the blocking peptide now not bind towards the epitope about the focus on protein. By evaluating the staining within the blocked antibody vs . the antibody alone, one can see which staining is certain.

Concentrate on Information and facts Cyclin E belongs to the extremely conserved cyclin loved ones, whose associates are characterized by a spectacular periodicity in protein abundance throughout the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins show unique expression and degradation patterns which add to get more info your temporal coordination of every mitotic occasion. Cyclin E sorts a fancy with and functions to be a regulatory subunit of CDK2, whose action is required for mobile cycle G1/S transition. This protein accumulates for the G1-S stage boundary and is degraded as cells progress through S period.

The measurement of protein phosphorylation is a great tool for measuring the modulation of receptor activation by both antibodies...

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